国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2190HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

簡要描述:HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2190
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:2352

產(chǎn)品分類

Product Category

詳細(xì)介紹

HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2

Depositors:RA Zager

Biosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: kidney, cortex
Tissue: proximal tubule
Cell Type: human papillomavirus 16 (HPV-16) transformed


Cellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HK-2 細(xì)胞,人腎近曲小管上皮細(xì)胞

Receptors:epidermal growth factor (EGF), expressed

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 9

D16S539: 11,12

D5S818: 12

D7S820: 10,11

THO1: 9

TPOX: 8,9

vWA: 17,18



Age:*****

Gender:male

Comments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney.

The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes.

The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2.

Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078).

Virus produced by the PA317 cells was used to transduce primary PTCs.

Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones.

The cell line appears to be derived from a single cell based on Southern and FISH analysis.

The E6/E7 genes are present in the HK-2 genome as determined by PCR.

The cells retain a phenotype indicative of well differentiated PTCs.

They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin.

The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase.

HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone.

The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen.

HK-2 cells are anchorage dependent.

The cells will not grow in methylcellulose, soft agar or suspension.

HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
精品无码久久久久久尤物| 俄罗斯美女视频| 国产精品沙发午睡系列99| 粗大的内捧猛烈进出| 西西人体WWW大胆高清| 老扒夜夜春宵第一部| 人妻丝袜无码国产一区| 特黄熟妇丰满人妻无码| 女教师の爆乳bd在线观看| 国产精品国产三级国产AV中文| 人妻妺妺窝人体色WWW聚色窝| 教子做爰xxxx| 9999久久久久精品无码| 国产人妻777人伦精品hd| 国产乱人伦偷精品视频不卡| 中文字幕无码亚洲字幕成a人蜜桃| 欧美性生交a片免费看| 欧美日韩国产精品| 久久久久亚洲精品无码网址| 啊灬啊灬啊灬快灬深视频无遮掩| 亚洲AV日韩AV永久无码色欲| 无码av人妻精品一区二区三区抖音 | 国产JIZZ中国JIZZ免费看| 99久久久国产精品免费蜜臀| 色噜噜狠狠色综合日日| 久久久综合精品一区二区三区| 性色AV一区二区三区| 随时都能干的校园运动会| 午夜DJ视频免费观看高清| 公与3个熄乱理在线播放| 亚洲香蕉成人AV网站在线观看| 成人免费电影网站| 富婆被大肉楱征服小说| 在线观看伦理片| 无码日韩人妻精品久久蜜桃| 老师含紧一点h边做边走视频动漫| 亚洲国产精品福利片在线观看| 国产精品亚洲ΑV天堂无码| 驯服人妻hd中字日本| 最近的最新的中文字幕视频| 50岁退休熟女露脸高潮|