国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2549Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2549
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1382

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2549™    Price:$417.00
Designations:Panc 03.27Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras + (wild-type)Antigen Expression:MHC class I +; MHC class II - [50655] Blood type A; Rh+DNA Profile (STR):Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 8,13 THO1: 6 TPOX: 8,9 vWA: 16,17Age:65 yearsGender:femaleEthnicity:WhiteComments:Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                               Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseDoubling Time:37.5 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-20200.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101Cell culture tested DMSO:ATCC 4-XReferences:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
精品人妻中文无码AV在线| 婷婷丁香五月| 国产SM重味一区二区三区 | 公与憩止痒小说400章| 精品少妇AY一区二区三区| 久久久久久精品国产亚洲| 公交车大龟廷进我身体里| 色国产精品一区在线观看| 中国妇被黑人xxx猛交| 色欲av| 国产 在线 | 日韩| 女人扒下裤让男人桶到爽| 英语老师解开裙子坐我腿中间 | 无翼鸟邪恶漫画| 日产亚洲一区二区三区| 男男情趣玩具play高h| 免费人成视频x8x8入口观看大| 最近中文字幕完整视频高清1 | 添女人下边视频全过程| 久久99国产精品成人| 久久久久久国产精品无码下载| 五十路熟女人妻一区二区| 欧美视频在线| 国产精品99久久久久久宅男| 精品人妻AV区波多野结衣| 日本a片| 无人区一码二码三码四码区| A性色生活片久久毛片牛牛| 久久精品国产精品亚洲精品| 热久久成人国产精品| 男女多p混交群体交乱a片小说| 美女脱内衣让男生揉摸的视频| 寡妇两腿间黑黑的毛毛是什么| 最近2019年中文字幕完整版免费| 精品人无码一区二区三区| 亚洲国产精品毛片AV不卡在线 | 汽车上开两个的花苞| 把腿抬高我要添你下面小说| 尤物视频在线观看| 久久精品国产亚洲AV香蕉| 国产日韩a视频在线播放视频|