国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2549Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2549
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1382

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2549™    Price:$417.00
Designations:Panc 03.27Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras + (wild-type)Antigen Expression:MHC class I +; MHC class II - [50655] Blood type A; Rh+DNA Profile (STR):Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 8,13 THO1: 6 TPOX: 8,9 vWA: 16,17Age:65 yearsGender:femaleEthnicity:WhiteComments:Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                               Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseDoubling Time:37.5 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-20200.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101Cell culture tested DMSO:ATCC 4-XReferences:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
岳今晚让我玩个够肥水一体探岳体| 人妻AⅤ无码一区二区三区| 破产姐妹第五季| 日本大片两个人免费观看| 精品无码久久久久久尤物| 午夜爽喷水无码成人18禁三级| 色综合久久久久久久久五月| 狂躁女人双腿流白色液体| 扒开老师双腿猛进入白浆小说| 亚洲AV无码久久精品狠狠爱浪潮 | av无码久久久久久不卡网站 | 亚洲国产成人av在线播放| 日本真人做爰免费的视频| 国产精品嫩草影院AV| 野花日本韩国免费视频6| 亚洲国产一区二区三区| 久久ER99热精品一区二区| 日韩av无码| 帅气体育生gary网站mv视频| 麻豆人妻少妇精品无码区| 欧美艳星NIKKI激情办公室| 又白又大的奶头a片免费| 娇小白人女vs巨大黑迪克| 夜夜爽妓女8888888视频| 精品成品国色天香卡一卡二| 看片神器免费| 高潮VPSWINDOWS国产乱| 嫖妓丰满肥熟妇在线精品| 午夜无码A级毛片免费视频| 99精品免费久久久久久久久日本 | 被老外添嫩苞添高潮np视频| 婷婷色国产偷v国产偷v| 亚洲AV色香蕉一区二区三区| 国产亚洲色婷婷久久99精品| CAOPORN免费视频国产| 公交车伦流澡到高潮hnp| 国产高潮流白浆啊免费a片动态| 欧美vivodeshd| 香港三级伦在线播放| 国产亚洲精品精品精品| 亚洲日本一线产区二线产区|