国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2547Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

簡(jiǎn)要描述:Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2547
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:1577

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞

ATCC® Number:CRL-2547™    Price:$338.00
Dsafety Level:1Shipesignations:Panc 10.05Depositors:EM JaffeeBioped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 10.05細(xì)胞,人胰腺腺癌細(xì)胞Isolation:Isolation date: 1992Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II -DNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9,12 D5S818: 13 D7S820: 8,9 THO1: 6,9.3 TPOX: 11 vWA: 16Age:*****Gender:maleEthnicity:WhiteComments:Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma. Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 40%. [50655] The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558).Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Temperature: 37.0°CSubculturing:Protocol:                        Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:19.2 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020derived from same individual:ATCC CRL-2558References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
综合欧美五月丁香五月| AV电影在线观看| 99久久久无码国产精品免费砚床| 大山里性混乱生活| free性满足vide0shd| 人妻少妇精品久久| 亚洲精品中字中出无码| 欧美成人片一区二区三区| 性色AV蜜臀AV人妻无码| 女性私密部位粉嫩| 久久国产加勒比精品无码| 色喜国模私密浓毛私拍人体图片| 日韩无码av| 免费av在线观看| 久久香蕉超碰97国产精品| 女性私密部位粉嫩| 清明节的来历和风俗| 久久亚洲AV永久无码精品| 欧美熟妇浓毛大bbw| 久久天天躁夜夜躁狠狠85麻豆| 玩弄丰满人妻大bbwbbw| 无码欧精品亚洲日韩一区| 电击惩罚花蒂榨乳调教小说| 先锋AV资源| 大炕上大战白胖老妇| 亚洲AV无码乱码精品国产福利| 免费国偷自产拍精品视频| 日本XXXX裸体XXXX免费| 女性私密整形视频| 亚洲精品无码专区| 公与淑婷厨房猛烈进出| 最近2019年中文字幕无吗| 国产精品久久国产精麻豆99网站| 糙汉lvl奶瘾古言| 寡妇大j8又粗又大| AV无码天堂一区二区三区| 我和小堂妺的第一次| 亚洲AV乱码一区二区三区林ゆな| 两个女人互添下身高潮| 日本人妻巨大乳挤奶水app| 女厕脱裤撒尿大全视频|