国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2553Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

簡要描述:Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2553
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:2090

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2553™    Price:$417.00
Designations:Panc 02.03Depositors:EM JaffeeBiosafety Level:1edium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Shipped:frozenMMorphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II - [50655]DNA Profile (STR):Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 9,10 THO1: 6 TPOX: 9,12 vWA: 17Age:70 yearsGender:femaleEthnicity:WhiteComments:Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 100%. [50655]Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                    Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:28.2 hrs [50655]Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
婷婷人人爽人人爽人人片| 高清毛片aaaaaaaaa片| 少妇性活bbbbbbbbb四川| 玉蒲团夜宵魂免费观看| 久久亚洲色一区二区三区| 国产精品污WWW一区二区三区| 男s严厉调教女m小说| 久久夜色精品国产嚕嚕亚洲AV| 欧美老熟妇欲乱高清视频| 男人一边吻奶边挵进去免费软件| 最近韩国日本免费观看MV| 国产在视频线精品视频| 国产美女在线精品免费观看| 两个人看的www视频免费完整版| 粗一硬一长一进一爽一A级| 我和子发生了性关系视频| 国产精品高潮呻吟久久AV无码| 美国人物动物交互入口复制| 美人被强行糟蹋np 各种play| 国产综合久久久久久鬼色| 国产一区二区三区播放心情潘金莲 | 熟妇人妻中文字幕| 名门嫡姝-213大h慎入| 久久人妻无码一区二区| gogo色少妇人体| 亚洲∧V久久久无码精品| 女人19水真多毛片学生| 人妻无码中文字幕免费视频蜜桃| 人妻少妇久久中文字幕| 成人免费视频在线观看| 国产又大又粗又长硬又紧又爽| 人妻系列无码专区久久五月天| 总被室友玩屁股(h)男男| 天天狠天天透天干天天怕∴| 国产精品久久久久久妇女| 伊人www22综合色| 少妇把腿扒开让我添69动态图| 58同城网招聘找工作| 国产又猛又黄又爽| 韩国三级日本三级美三级| 国产成人A∨激情视频厨房|