国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2741HBE135-E6E7人支氣管上皮細(xì)胞

HBE135-E6E7人支氣管上皮細(xì)胞

簡要描述:HBE135-E6E7人支氣管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2741
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:3196

產(chǎn)品分類

Product Category

詳細(xì)介紹

HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.



細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells     under an inverted microscope until       cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping, do not agitate the cells     by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh serum-free growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37°C.


Subc*tion Ratio: 1:3 to 1:4

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994.

培養(yǎng)條件:Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat.  17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
肉多荤文高h羞耻玩弄校园| 欧美 国产 综合 欧美 视频| 好硬好大好爽18禁免费看| 久久久久久久女国产乱让韩| 校花玉腿缠腰娇喘迎合| 国产精品一区二区毛卡片| 成人在线视频| 欧美人与动性XXXXX杂性| 一本加勒比hezyo东京热高清| 國産麻豆av天美傳媒| 久久久精品日本一区二区三区| 欲插爽乱浪伦骨| 少洁白妇无删减全文阅读| 亚洲AV日韩AV永久无码下载| 做床爱视频真人版无遮挡免费| 精品一区二区三人妻视频| 精品熟女少妇AV免费观看| 最近高清中文字幕免费MV| 欧美mv日韩mv国产网站| 无码精品视频一区二区三区| 亚洲精品第一国产综合野草社区 | 中文字幕亚洲乱码熟女在线萌芽| 波多野结衣中文| 国产人妻无码一区二区三区不卡| 无码国产精品一区二区免费虚拟VR | 双xing挨cao日常美人多汁| 久久泄欲网| ぱらだいす天堂中文网.WWW | 狠狠躁日日躁夜夜躁2022麻豆 | 亚洲欧洲国产综合AV无码久久| 日韩无码av| 亚洲中文国产最新发布| 怀孕妇女bbwbbw| 久久久久久无码大片a片| 国产免费观看黄AV片| 丰满熟妇乱又伦在线无码视频| 美女张开腿让男人桶爽| 激情综合色综合久久综合| 天天躁日日躁狠狠躁AV| 丰满多毛的大隂户毛茸茸| 黄金网站app在线观看|