国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>EA.hy926人臍靜脈細(xì)胞融合細(xì)胞

人臍靜脈細(xì)胞融合細(xì)胞

簡要描述:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:EA.hy926
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:4389

產(chǎn)品分類

Product Category

詳細(xì)介紹


ATCC® Number: CRL-2922™ Price:

Designations:

EA.hy926

Depositors: CS Edgell

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: endothelial

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Source: Tissue: somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression: Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Comments: The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.

Atmosphere: air, 95; carbon dioxide (CO2), 5

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

CRL-2922 EA.hy926

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

6.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

7.Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.

Interval: Twice a week

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Preservation: Freeze medium: Complete Growth medium, 95; DMSO, 5

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

CRL-2922 EA.hy926

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC 30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402

References: 92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

CRL-2922 EA.hy926

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270





























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
最近的最新的中文字幕视频| 高潮又爽又无遮挡又免费 | 亚洲A∨无码一区二区| 少年汁水四溅捣出白沫h| 波多野结衣中文字幕一区二区三区 | 国产热の有码热の无码视频| 一个添下面两个吃奶把腿扒开| 调教yin荡女友系列合集| 熟女俱乐部五十路六十路AV | 特级做A爰片毛片免费看无码| 日日噜噜夜夜狠狠VA视频| chinese山西老熟女bbw| 337p人体粉嫩胞高清大图av| 亚洲熟妇少妇任你躁在线观看无码| 麻豆传煤入口免费进入2023| 寡妇大j8又粗又大| 国产伦精品一区二区三区免费| 国产精品自在线拍国产电影 | 粉嫩小泬无遮挡久久久久久| 日本AAA少妇高潮免费中国| 日韩电影免费在线观看网站| 国产精品天天看天天做夜夜爽| 国产精品久久久久久AV| 亚洲午夜无码久久久久软件| 精品国产乱码久久久人妻| japanese丰满人妻hd| 新婚妻子1-3部20章| 久久久久亚洲精品男人的天堂 | 蜜臀AV免费一区二区三区 | 国产精品免费无遮挡无码永久视频| 中文字幕乱码无码人妻系列蜜桃| 欧美又黄又嫩大片a级| 精品久久久久久亚洲偷窥一区| 粗大的内捧猛烈进出a片| 熟妇人妻AV无码一区二区三区| 国产啪亚洲国产精品无码| 99久久久无码国产精品试看| 日本爽爽爽爽爽爽在线观看免| 性猛交╳xxx乱大交| 精品2022露脸国产偷人在视频| 日本16岁rapper|