国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>HUV-EC-C人臍靜脈內(nèi)皮細(xì)胞

人臍靜脈內(nèi)皮細(xì)胞

簡要描述:CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):HUV-EC-C
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:2738

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞

ATCC® Number: CRL-1730™ Price: $280.00

Designations: HUV-EC-C

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: endothelial

CRL-1730 HUV-EC-C 人臍靜脈內(nèi)皮細(xì)胞

Source: Organ: umbilical vein

Tissue: vascular endothelium

Disease: normal

Cell Type: endothelial

Cellular Products: factor VIII [23284]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (technology from amaxa)

Tumorigenic: No

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16

Cytogenetic Analysis: This is a hypodiploid human cell line. The modal chromosome number

was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments: Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: Two to three times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682

29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915

33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443



CRL-1730 HUV-EC-C
























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日韩性做爰免费a片aa片| 国内老熟妇对白HDXXXX | 亚洲国产精品久久久久秋霞小 | 欧美人与性动交Α欧美精品| 和六十老女人高潮多次| 久久精品熟一区二区三区| 激烈 痉挛 抽搐 潮喷 MP4| 极品白嫩小泬10p| 毛茸茸性XXXX毛茸茸毛茸茸| 老妇性hqmaturetube| 亚洲AV永久中文无码精品综合 | 富婆被大肉楱征服小说| 久久精品国产亚洲AV蜜臀色欲| 真人插B免费视频播放| 又大又黄又粗又爽的免费视频| 屁股眼扒开给客人打烂作文 | 熟妇人妻中文字幕| 女厕偷拍txxxxxxx视频| 国产草草影院CCYYCOM| 韩国伦理电影网站| 摄像头东北对白清晰| 欧美老妇疯狂XXXXBBBB| 唐舞桐灌满jing液h斗罗h| bl道具play珠串震珠强迫| 久久综合亚洲色HEZYO国产| 韩国三级日本三级少妇| 9lporm自拍视频区九色| 被夫の上司に犯波多野结衣| 老熟女五十路乱子交尾中出一区| 东北大坑乱全集目录无删| 欧美黑人粗暴多交高潮水最多| 麻豆av福利av久久av| 国产L精品国产亚洲区久久 | 性疼痛tube小坳交hd| 五级黄高潮片90分钟视频| 少妇仑乱A毛片无码| 老板含着她的花蒂啃咬高潮的视频| 天天做天天摸天天爽天天爱| 337p粉嫩大胆色噜噜噜| 乱肉黄蓉合集500篇| 欧美精产国品一二三产品工艺|