国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>NCI-H226人肺癌細(xì)胞株

人肺癌細(xì)胞株

簡(jiǎn)要描述:CRL-5826 NCI-H226 人肺癌細(xì)胞株 ,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):NCI-H226
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:2030

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-5826 NCI

-H226 人肺癌細(xì)胞株ATCC® Number: CRL-5826™

Designations: NCI-H226 [H226]

Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: Epithelial

CRL-5826 NCI-H226 人肺癌細(xì)胞株

Source: Organ: lung

Disease: squamous cell carcinoma; mesothelioma [23570]

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233.

Isolation: Isolation date: March, 1980

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 13,14

D16S539: 9,12

D5S818: 11,12

D7S820: 8,10

THO1: 8,9.3

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: modal number = 47; range = 32-88; del(p25)

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to3 days

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..


CRL-5826 NCI-H226


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
色综合久久中文字幕无码| 三上悠亚ssni绝顶を教え込ま| 又湿又紧又大又爽A视频国产| 大内密探零零性性| 精品人体无码一区二区三区| 茄子视频APP色版 永久免费| 少妇高h肉辣全集目录| 亚洲欧洲无码AV电影在线观看| 国产精品久久久久久久久免费| 年轻老师2韩国手机在线| 国产精品丝袜黑色高跟鞋| 婷婷综合另类小说色区| 嫩bbb槡bbbb槡bbbb| 青楼男妓h高潮啊哈男男| 久久久精品国产SM最大网站| 国内精品九九久久久精品| 少妇人妻av| 被狼交的女人(h)| 男女啪啪流出白色液体| 国产古装妇女野外a片| 大战熟女丰满人妻av| 囯产精品一区二区三区线| 亚洲男人天堂| 日本免费a片| 亚洲无码在线播放| 国产精品污WWW一区二区三区| 国产精品白浆无码流出| 日本美女视频| 国产在线拍揄自揄拍无码视频| 国产偷人爽久久久久久老妇app| 漂亮人妻洗澡被公强 日日躁| 免费无码又爽又高潮视频| 人妻少妇精品无码专区二区| h双腿涨灌捆绑play慎入| 精品少妇高潮蜜臀涩涩av| 国产强伦姧人妻毛片| 亚洲国产精品18久久久久久| 熟妇人妻无乱码中文字幕| 最近最新高清中文字幕| 欧美成人猛片AAAAAAA| А√天堂8资源中文在线|