国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>NCI-H226人肺癌細(xì)胞株

人肺癌細(xì)胞株

簡(jiǎn)要描述:CRL-5826 NCI-H226 人肺癌細(xì)胞株 ,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):NCI-H226
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:2030

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-5826 NCI

-H226 人肺癌細(xì)胞株ATCC® Number: CRL-5826™

Designations: NCI-H226 [H226]

Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: Epithelial

CRL-5826 NCI-H226 人肺癌細(xì)胞株

Source: Organ: lung

Disease: squamous cell carcinoma; mesothelioma [23570]

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233.

Isolation: Isolation date: March, 1980

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 13,14

D16S539: 9,12

D5S818: 11,12

D7S820: 8,10

THO1: 8,9.3

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: modal number = 47; range = 32-88; del(p25)

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to3 days

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..


CRL-5826 NCI-H226


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
精品无码国产污污污免费网站| 成人做爰高潮a片免费视频| 你是我的城池营垒全集免费观看| 国产午夜视频在线观看| 两根一起进去好紧好涨| 日本免费a片| 性少妇jealousvue成熟| 人妻夜夜爽天天爽爽一区| 最近最新高清中文字幕| 国产V亚洲V天堂无码网站| 无码国产精品一区二区免费16| 麻豆国产一区二区三区四区| 斯文教授×貌美学生1v1h| 国内精品乱码卡一卡2卡三卡| 亚洲AV无码国产一区二区| 国产肥熟女视频一区二区三区| 性色AV蜜臀AV人妻无码| 亚洲av成人片无码www| 国产真实乱人偷精品视频| 黑人勃起太大进不去| 亚洲国产成人片在线观看无码| 玩弄放荡人妻少妇系列视频| 脱岳裙子从后面挺进去视频 | 亚洲AV午夜成人片精品网站 | 闺蜜和我被黑人4p到惨| 美女黄网站视频免费视频软件| 偷朋友人妻系列h文| 久久AV无码ΑV高潮ΑV喷吹| 欧美三级在线播放| 全黄H全肉短篇禁乱NP慕浅浅| 嗯啊灬别停啊灬用力灬快| 四虎成人精品国产永久免费无码| 日产无人区一线二线三线HAV | japanese日本护士xxx| 把少妇弄高潮了WWW| 国产精品无码一本二本三本色| 亚洲男同帅gay片在线观软件 | 777久久人妻少妇嫩草av| 亚洲国产AV一区二区三区| 无码国产精品一区二区免费16| 99亚洲精品自拍av成人软件|