国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1676 WM-266-4 人黑素瘤細(xì)胞

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

簡(jiǎn)要描述:CRL-1676 WM-266-4 人黑素瘤細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪  問  量:1973

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Designations: WM-266-4

Depositors: M Herlyn

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Cellular Products: proteoglycan antigen characteristic of melanoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-1676 WM-266-4 人黑素瘤細(xì)胞

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12,13

D16S539: 11,12

D5S818: 13

D7S820: 8

THO1: 7,9

TPOX: 8,11

vWA: 15,17

Cytogenetic Analysis: The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.

Age: 58 years

Gender: female

Comments: The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma.

Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-1675



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
东北粗口国产床| 亚洲VA久久久噜噜噜久久天堂 | 变态捡到女婴h养成调教| 精品久久久无码中文字幕| 丝袜 中出 制服 人妻 美腿| 久久人妻熟女中文字幕av蜜芽| 亚洲AⅤ无码专区在线观看Q| 当着夫的面被夫上司玩弄| 亚洲AV午夜精品一区二区三区| 欧美大屁股眼子xxxxx视频| 精品无码AV一区二区三区| 天天躁日日躁狠狠躁AV麻豆 | av免费在线观看| 日韩AV无码一区二区三区不卡| 日本三级在线播放| A级毛片在线观看| 无码播放一区二区三区| 波多野结衣的电影| 国产老熟女精品一区| 精品少妇爆乳无码av无码专区| 男女啪啪做爰高潮全过有多少姿势| 亚洲日本一线产区二线产区| 国产性色AV高清在线观看| 永不迷路-黑料正能量在线| 久久亚洲AV无码精品色午夜麻豆| 99久久国产精品人妻无码| 掀开奶罩边躁狠狠躁软学生电影| 图片区小说区激情区偷拍区| 全免费A级毛片免费看无码| 太长又太大又太粗太疼了| 国产刺激对白 国产情侣| 囯产精品一品二区三区| 亚洲 自拍 另类 欧美 综合| 国内揄拍国内精品人妻试看| 男生和女生一起打扑克| 握住校花两团雪乳高h| 亚洲午夜无码AV毛片久久| 国产成人无码一区二区三区在线| 公交车上内裤滑进去了会怎么样| 国产老熟女精品一区| 久久久777天天躁狠狠躁av|