国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2119HPAC 人胰腺癌細(xì)胞

HPAC 人胰腺癌細(xì)胞

簡要描述:CRL-2119 HPAC 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2119
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-26
  • 訪  問  量:1860

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2119 HPAC 人胰腺癌細(xì)胞

ATCC® Number: CRL-2119™ Price: $323.00

Designations: HPAC

Depositors: WR Gower

Biosaum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreasfety Level: 1

Shipped: frozen

Medium & Ser

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2119 HPAC 人胰腺癌細(xì)胞

Isolation: Isolation date: 1985

Receptors: epidermal growth factor (EGF), expressed

glucocorticoid, expressed

epidermal growth factor (EGF); glucocorticoid

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,10

D5S818: 12

D7S820: 10,12

THO1: 9.3

TPOX: 10,11

vWA: 15,17

Cytogenetic Analysis: modal number = 61

Age: 64 years

Gender: female

Ethnicity: Caucasian

Comments: HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.

HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha).

Cell growth is suppressed by dexamethasone and other glucocorticoids.

HPAC cells are positive for keratin and negative for vimentin and chromogranin A.

Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.

Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.

In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.

HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 41 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 22987: Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.



CRL-2119


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美人与物VIDEOS另类| 无遮挡国产高潮视频免费观看| 国产精品国产三级国产AV主播| 精品无人区无码乱码毛片国产| 男人撕开奶罩揉吮奶头gif | 扒开双腿抽打花蒂惩罚室 | 无码日韩精品一区二区人妻| 长篇交换高h肉辣全集目录 | 老师黑色双开真丝旗袍| 无码视频一区二区三区| 白女人荫蒂高潮视频| 国产精品日产欧美久久久| 特级做a爱片久久久久久| 无码任你躁久久久久久| 天堂资源最新在线| 国产香港明星裸体XXXX视频| 交换俱乐部娇妻奶呻吟啊视频| 老师在办公室被躁在线观看| 玩弄六个女邻居| 亚洲AV人无码综合在线观看| 国产精品不卡一区二区| 国产成人无码AA精品一区| 偷拍农民工嫖妓bbbbb| 一本一道色欲综合网中文字幕| 国产精品久久久久精品艾秋| 性一交一乱一乱一视频| 亚洲性色AV私人影院无码| 70歳の熟女セックス合集| 狠狠躁18三区二区一区| 天堂8中文在线最新版官网| 美国人物动物交互入口复制| 浪荡人妻共32部黑人大凶器电影| 人妻无码一区二区三区TV| 东北熟妇啪啪流白浆| 名器紧致h拔不出来| 国产精品美女久久久免费| 把腿张开开被添死去活来| 极品少妇高潮啪啪av无码| 日韩精品免费一线在线观看| 日本av在线观看| 扒开粉嫩小泬舌头伸进去|