国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2119HPAC 人胰腺癌細(xì)胞

HPAC 人胰腺癌細(xì)胞

簡要描述:CRL-2119 HPAC 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2119
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-26
  • 訪  問  量:1860

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2119 HPAC 人胰腺癌細(xì)胞

ATCC® Number: CRL-2119™ Price: $323.00

Designations: HPAC

Depositors: WR Gower

Biosaum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreasfety Level: 1

Shipped: frozen

Medium & Ser

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2119 HPAC 人胰腺癌細(xì)胞

Isolation: Isolation date: 1985

Receptors: epidermal growth factor (EGF), expressed

glucocorticoid, expressed

epidermal growth factor (EGF); glucocorticoid

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,10

D5S818: 12

D7S820: 10,12

THO1: 9.3

TPOX: 10,11

vWA: 15,17

Cytogenetic Analysis: modal number = 61

Age: 64 years

Gender: female

Ethnicity: Caucasian

Comments: HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.

HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha).

Cell growth is suppressed by dexamethasone and other glucocorticoids.

HPAC cells are positive for keratin and negative for vimentin and chromogranin A.

Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.

Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.

In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.

HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 41 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 22987: Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.



CRL-2119


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
扒开双腿疯狂进出爽爽爽动态照片| 天天AV天天翘天天综合网| 粉嫩名器少妇名器| 久久天天躁狠狠躁夜夜AV| 好男人资源在线看免费的| 午夜18禁试看120秒男女啪啪| 性xxxxfreexxxxx| 免费精品无码AV片在线观看| 久久精品国产亚洲av香蕉高清| 少妇大荫蒂被巨大爽爽大| 亚洲国产天堂久久综合| 丰满人妻中伦妇伦精品app| 95免费视频在线观看| 亚洲AV无码专区国产乱码不卡| 午夜福利视频| 公交车上拨开丁字裤进入电影| 久久久久亚洲精品无码网址| 扒开腿狂躁女人视频免费| AV在线亚洲欧洲日产一区二区| 我在ktv被六个男人玩一晚上| 男女18禁啪啪无遮挡激烈网站 | 无码国产69精品久久久久孕妇| 金梅瓶在线观看| 日本少妇被黑人猛CAO| 欧美人与牲动交XXXX| 国产小受呻吟av视频在线观看| 久久强奷乱码老熟女网站| 色噜噜狠狠狠综合曰曰曰| 玩弄人妻少妇500系列| 亚洲 自拍 另类小说综合图区| 国内精品乱码卡一卡2卡三卡| 韩国三级| 小sb是不是欠c流了那么多| 国外AV无码精品国产精品| 性XXX欧美老妇5060.70| 女性私密紧致按摩| 黑人60厘米全进去了| 国产手机拍视频推荐2023| 巨大黑人极品VIDEOS精品| 久激情内射婷内射蜜桃| 免费AV一区二区三区|