国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1503STO 小鼠胚成纖維細(xì)胞系

STO 小鼠胚成纖維細(xì)胞系

簡要描述:CRL-1503 STO 小鼠胚成纖維細(xì)胞系
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1503
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2193

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系 的詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系

ATCC® Number:CRL-1503™  Price:$289.00
Designations:STO

Depositors:G Martin

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Organ: embryo
Strain: SIM
Cell Type: fibroblast


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1503

Isolation:Toronto Ontario, Canada

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Age:embryo

Comments:The STO cell line was derived by A. Bernstein, Ontario Cancer Institute, Toronto, Canada from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. The cell line is used routinely to prepare feeder layers by irradiation or mitomycin C treatment. Such populations are then employed for maintenance of stock teratocarcinoma stem cells (see ATCC CRL-1535 and CRL-1566) in the undifferentiated state.The cells have been tested and found negative for ectromelia virus (mousepox).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended
      Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

irradiated to be used as feeder cells:ATCC 56-X



References:1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

21874: . Teratomas and differentiation. New York: Academic Press; 1975.

22387: . . Cell 6: 467-474, 1975.

22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
精品国产乱码一区二区三区| 日本japanese丰满白浆| 12末发育娇小性色xxxx| 一女被多男玩喷潮视频免费看| 人人妻人人澡人人爽人人精品蜜桃 | 极品粉嫩小泬白浆20pa片| 99精产国品一二三产区区| 亚洲AV影院一区二区三区| 日本公妇乱偷中文字幕| 精品国产一区二区三区久久影院| 下一轮油价调整预测| 娇小1213╳YⅩ╳毛片| 屁股眼扒开给客人打烂作文 | 欧美xxxxxoo大尺度| 久久人妻少妇嫩草AV无码专区| 和子发生了性关系的免费视频| 国产香蕉97碰碰久久人人| 日本无码sm凌虐强制m字开腿| 国产精品久久久久9999赢消| 草草浮力影院| 国产成人精品三级麻豆| 国产真实乱了露脸在线观看| 久久久久黑人强伦姧人妻| 韩国午夜理伦三级在线观看中文版| 寡妇两腿间黑黑的毛毛是什么| 亚洲一久久久久久久久| 暴躁老阿姨csgo| 色窝窝无码一区二区三区| 男女啪啪做爰高潮免费网站| 亚洲爆乳无码一区二区三区| 成人精品一区二区三区不卡免费看| 18禁黄网站男男禁片免费观看| 国模冰冰02[150p]色综合| 日本a级作爱片| 成人电影免费看| 日本大片两个人免费观看| 久久人人爽人人爽人人AV| 欧美freesex黑人又粗又大| 老司机福利导航| 精品乱子伦一区二区三区| 扒开老师双腿猛进入白浆小说|