国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1503STO 小鼠胚成纖維細(xì)胞系

STO 小鼠胚成纖維細(xì)胞系

簡要描述:CRL-1503 STO 小鼠胚成纖維細(xì)胞系
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1503
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2193

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系 的詳細(xì)介紹

CRL-1503 STO 小鼠胚成纖維細(xì)胞系

ATCC® Number:CRL-1503™  Price:$289.00
Designations:STO

Depositors:G Martin

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast


Source:Organ: embryo
Strain: SIM
Cell Type: fibroblast


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1503

Isolation:Toronto Ontario, Canada

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Age:embryo

Comments:The STO cell line was derived by A. Bernstein, Ontario Cancer Institute, Toronto, Canada from a continuous line of SIM mouse embryonic fibroblasts. The population which was selected for 6-thioguanine and ouabain resistance is sensitive to HAT medium and is HPRT negative. The cell line is used routinely to prepare feeder layers by irradiation or mitomycin C treatment. Such populations are then employed for maintenance of stock teratocarcinoma stem cells (see ATCC CRL-1535 and CRL-1566) in the undifferentiated state.The cells have been tested and found negative for ectromelia virus (mousepox).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:10 is recommended
      Medium Renewal: 2 to 3 times per week



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

irradiated to be used as feeder cells:ATCC 56-X



References:1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416

21874: . Teratomas and differentiation. New York: Academic Press; 1975.

22387: . . Cell 6: 467-474, 1975.

22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
最新国产の精品合集bt7086| 肉体撞击声噗嗤噗嗤水声| 九色丨porny丨蝌蚪视频| 在床上拔萝卜又疼又叫什么病| 日本丰满少妇高潮呻吟| 一本一道久久A久久精品综合| 白嫩的18sex少妇hd| 久久人人爽人人人人爽AV| 日本免费无码一区二区到五区| 浴室人妻的情欲hd三级国产| 人人妻人人爽人人做夜欢视频九色| 女公浴室xxx偷窃www| 老妇人高清在线观看免费版| 中文字幕乱码中文| 亚洲国产精品久久久久蜜桃网站 | 午夜不卡久久精品无码免费| hkdoll玩偶在线无码国产| 久久午夜无码鲁丝片午夜精品 | 精品久久99麻豆蜜桃66| 天天摸夜夜躁夜夜无码小视频| 国产精品VA在线播放我和闺蜜| 真人无遮挡猛进猛出视频| 老熟女重囗味HDXX70星空| 国模吧无码一区二区三区| 国产精品乱码人妻一区二区三区| 天天躁了天天躁了天天躁| 欧美激情一区二区三区在线| 乡下人产国偷v产偷v自拍| 国产强被迫伦姧在线观看无码 | 极品yin荡人妻合集h| 国产精品亚洲ΑV天堂无码| 我和子发生了性关系视频| 精品少妇无码AV无码专区| 精品人妻久久久久久888| g0g0全球高清大胆人休艺术网| 精品久久香蕉国产线看观看亚洲 | 色综合久久无码五十路人妻 | 日韩人妻精品一区二区三区视频| 三上悠亚人妻中文字幕在线 | 国产成人无码AA精品一区| 教子做爰xxxx|