国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>CRL-1444A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞

A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞

簡要描述:CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-1444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2479

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CRL-1444 A7r5 大鼠胸大動(dòng)脈平滑肌細(xì)胞的詳細(xì)介紹


ATCC® Number:CRL-1444™  Price:$275.00
Designations:A7r5

Depositors:W Carlisle

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:fibroblast

Source:Organ: aorta, thoracic
Strain: DB1X
Tissue: smooth muscle


Cellular Products:myokinase; creatine phosphokinase (muscle isoenzyme); myosin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1444

Applications:transfection host (Roche FuGENE® Transfection Reagents

technology from amaxa)



Age:embryo

Comments:The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase.

Muscle type CPK is synthesized after cell division has ceased.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301

CRL-1444




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
色老太bbwbbwbbw高潮| 亚洲日韩乱码一区二区三区四区 | 69精品丰满人妻无码视频a片| 国产男女爽爽爽免费视频| 国产专区一线二线三线品牌| 国产精品免费A V片在线观看| 欧美肥胖老太玩黑人| 久久嫩草精品久久久久精品| 女人被狂躁g点高潮喷水| 无码人妻精品一区二区三区9厂| 玩弄了裸睡少妇人妻野战| 寡妇大j8又粗又大| 精品无码久久久久久久久久| 草莓视频APP黄| 最新av网址| 被黑人伦流澡到高潮hnp动漫| 久久99久久精品免观看吃奶| 精品国产亚洲第一区二区三区| 美女直播全婐app免费| 日产精品一卡2卡三卡4乱码| 国产精品久久久久无码AV| 国精品无码一区二区三区在线| 亚洲AV无码AV日韩AV网站| 色婷婷五月| 12末发育娇小性色xxxx| 青青草97国产精品免费观看| 国产精品揄拍100视频| 香蕉久久人人爽人人爽人人片AV| 宿主每日被guan满的日常| www四虎最新成人永久网站| 日韩+国产| 黄金网站app在线观看| 欧美大屁股XXXX高跟欧美黑人| 亚洲AV无码久久久久网站蜜桃| 日日噜噜噜噜夜夜爽亚洲精品 | 亚洲精品无码午夜福利中文字幕| 麻豆传煤入口免费进入2023| 熟妇五十路六十路息与子| 亚洲精品久久久久一区二区三区| 青春草在线视频观看| 惩罚她给她用最粗的玉势h|