国产AⅤ激情无码久久久无码,天堂8中文在线最新版官网,99久久久国产精品免费蜜臀,欧美乱妇日本无乱码特黄大片

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

簡要描述:CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問  量:2388

產(chǎn)品分類

Product Category

詳細(xì)介紹


CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


ATCC® Number:CRL-2268™Price:$329.00
Designations:BE(2)-C

Depositors:JL Biedler

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:neuroblast
CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


Source:Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Restrictions:BE(2)-C was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. BE(2)C is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)BE(2)-C or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of BE(2)-C including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:Isolation date: November, 1972

DNA Profile (STR):Amelogenin: X

CSF1PO: 10

D13S317: 11

D16S539: 9,11

D5S818: 12

D7S820: 9,10

THO1: 6

TPOX: 11

vWA: 18



Age:2 years

Gender:male

Comments:BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.

BE(2)-C cells have a reported saturation density of greater than 5 X 10 exp5 cells/sq cm.

The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites).

Unlike the parent line, they generally do not detach and float.



Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase



Doubling Time:18 hrs

Related Products:recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2271
























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
xxxx18一20岁hd| 老头在厨房添下面很舒服| 艳妇臀荡乳欲伦交换h| 免费无码国产欧美久久18| 四虎成人精品国产永久免费无码| 日文中字乱码一二三区别在哪| 黄海川邱舒涵全文免费阅读| 国产成人涩涩涩视频在线观看 | 性做久久久久久免费观看| 久久99精品国产99久久6尤物| 95免费视频在线观看| 上司人妻互换HD无码中文| 杨思敏1—5集无删减在线观看| 第一次交换完美邻居| 久久久久无码精品国产AV蜜桃1| 边做边爱mp3在线播放免费观看 | 精品无码久久久久久久久| 强开美妇后菊哀嚎哭叫视频| 人人妻人人澡人人爽欧美二区| 黑人巨大精品欧美黑寡妇av免费 | 人人澡人人透人人爽| 被多个男人调教奶头玩奶头| 夜色88v精品国产亚洲av| 国产农村妇女毛片精品久久| 亚洲AV片在线观看| 男人一边吻奶边挵进去免费软件| 大秦赋全集1-78集免费观看| 曰韩无码无遮挡A级毛片| 年轻丰满的继牳4伦理| 久久亚洲精品无码AⅤ大香| 高雅人妻被迫沦为玩物| 青柠在线影院观看日本| 国产片+人+综合| 久久久久久久无码高潮| 国产成人免费ā片在线观看| free性开放小少妇| 小处雏一区二区三区精品视频| 无码精品人妻一区二区三区漫画 | 在线无码VA中文字幕无码| 日本最新男男gayjapan| 14表妺好紧没带套18分钟|